Thermo Fisher Scientific Osteopontin Polyclonal Antibody
Rabbit polyclonal antibody targeting human Osteopontin. Validated for WB and IHC-P. Recommended antigen retrieval with citrate buffer. Suitable for studying bone mineralization and cell adhesion. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from internal domain of human osteopontin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1077368 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is breast carcinoma tissue.
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors.
It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease, and transformation.
Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding Arg-Gly-Asp sequence modulates OPN function and binding properties.
Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors, providing a molecular niche with survival advantage and serving as a substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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