Thermo Fisher Scientific MMP19 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from the N-terminus of human MMP-19 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MMP19,uniProtId:Q99542-1,ncbiNodeId:9606,antigenRange:1,antigenLength:508,antigenImageFileName:PA5-32502_MMP19_Q99542-1_Rabbit.svg,antigenImageFileNamePDP:PA5-32502_MMP19_Q99542-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549968

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta tissue.

Target Information

MMP-19 (RASI-1, MMP-18) is a 508 amino acid peptide, originally isolated as an autoantigen from the synovium of a patient suffering from rheumatoid arthritis (RA). Its presence on the surface of activated PBMCs, TH1 lymphocytes, and Jurkat T lymphoma cells in the synovium of acute RA patients, suggests that MMP-19 plays a role in RA-associated joint tissue destruction. MMP-19 exists in the smooth muscle cells of the tunica media of large blood vessels of the RA patient, but not in the endothelial cell layer. Acutely inflamed tissue synovial capillaries strongly express MMP-19 in the cytoplasm. Regulated induction of MMP-19 in capillary endothelial cells during acute inflammation suggest a role in angiogenesis. MMP-19 is a single 2.7kb transcript found in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis, colon, and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.