Conveniently isolate AAV particles from AAV-producing cells using either the AAVPro Extraction Solution (for live cells only) or AAVpro Freeze-Thaw Extraction Buffer (All Serotypes) (for live and frozen cells).
Conveniently isolate AAV particles from AAV-producing cells using either the AAVPro Extraction Solution (for live cells only) or AAVpro Freeze-Thaw Extraction Buffer (All Serotypes) (for live and frozen cells).
AAVpro Extraction Solution, a reagent set for the extraction of AAV particles from producer 293T cells, increases AAV yield by at least three-fold. AAV particle isolation is typically performed using freeze-thaw or sonication methods. However, these methods are time consuming and require special equipment. The AAVpro Extraction Solution uses an improved method for AAV particle isolation; simply add the extraction solution to virus-producing cells and recover viral particles by centrifugation. In addition to a simple protocol, the extraction solution reduces host protein and nucleic acid contamination, yielding viral particle solutions that are ready for cell infection or further purification with the AAVpro Purification Kit.
NOTE: This product is included with our AAVpro helper-free systems.
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