Takara Ecotropic Receptor Booster

Takara Ecotropic Receptor Booster

The Ecotropic Receptor Booster is used to temporarily elevate the density of the ecotropic receptor protein mCAT-1 on the surface of any target cell that you wish to infect. This enables efficient transduction of human cells with ecotropic retrovirus or lentivirus that otherwise could only infect rodent cells. The technology also allows you to increase the infection efficiency of any cell type that is resistant to viral infection.

Recommended Products for Producing Ecotropic Pseudotyped Virus

• Ecotropic lentivirus — Lenti-X Packaging Single Shots (Ecotropic)
• Ecotropic retrovirus — Retro-X Universal Packaging System or EcoPack 2-293 Cell Line

How It Works

The Ecotropic Receptor Booster consists of a vial of concentrated exosome-like vesicles densely coated with the mCAT-1 receptor protein. When the booster vesicles are applied to your cells (10–20 µl per well of a 6-well plate, for approximately 2 hours), they fuse with the plasma membrane, resulting in an increased level of the receptor on the cell surface. Your target cells can then be more efficiently transduced with ecotropic pseudotyped lentivirus or retrovirus.