Thermo Fisher Scientific IL-1 beta Polyclonal Antibody, Biotin
Biotin으로 표지된 Thermo Fisher IL-1 beta 폴리클로날 항체. Human, Dog, Non-human primate 반응성. Western blot, IHC, ELISA에 적합. 4°C 단기, -20°C 장기 보관 가능. 염증성 사이토카인 IL-1β 검출에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Prepared by repeated immunizations with recombinant human IL-1β produced in E. coli. The recombinant 153 aa IL-1β is 17 kDa with the N-terminal amino acid at alanine 117, generated by IL-1β converting enzyme (ICE, caspase-1). |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion (Gel filtration) |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid; dilute only before immediate use.
- Heated to 56°C for 30 minutes. IgG preparation of whole rabbit serum purified by DEAE fractionation.
- Directed against mature 17 kDa human IL-1β; also detects primate IL-1β but not IL-1α.
- In ELISA, recognizes ~10% of non-denatured 31 kDa precursor IL-1β but primarily detects mature 17 kDa form.
- In immunoblots, denatured 31 kDa precursor IL-1β appears as 35 kDa band due to unfolding and exposure of epitopes.
- Detects 17 kDa band in supernatants and 35 kDa precursor band in lysates.
Target Information
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized as a 31 kDa inactive pro-form and cleaved into a 17 kDa active form via inflammasome activation and caspase-1 processing. IL-1β lacks a classical secretion signal and is released through an alternate mechanism. It signals through IL-1RI and IL-1RII receptors shared with IL-1α. IL-1β activity is modulated by IL-1 Receptor Antagonist (IL-1RA). Elevated IL-1β levels are linked to chronic inflammatory conditions, and IL-1β neutralizing antibodies have therapeutic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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