Thermo Fisher Scientific BDNF Monoclonal Antibody (3D9C5)
BDNF 단백질을 인식하는 Mouse monoclonal antibody (clone 3D9C5). Western blot, ICC/IF, ELISA에 적합. Protein G로 정제된 액상 항체로, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D9C5 |
| Immunogen | Purified recombinant fragment of human BDNF (aa 19–248) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898370 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor p75-NTR has also been demonstrated. BDNF promotes neuronal survival and differentiation and plays a critical role in memory formation and synaptic regulation. Expression of BDNF is reduced in Alzheimer’s and Huntington’s disease, and may also be involved in stress response and mood disorder regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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