Thermo Fisher Scientific BNIP3 Monoclonal Antibody (6A5F7)
BNIP3 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등에 사용 가능. 단백질 G로 정제된 액상형 제품이며, 4°C 또는 -20°C에서 보관. 인간 BNIP3 단백질(aa 50-155) 유래 면역원 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6A5F7 |
| Immunogen | Purified recombinant fragment of human BNIP3 (aa 50–155) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898373 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
NIP3 (BNIP3) belongs to the BCL2/adenovirus E1B 19 kDa-interacting protein family. It interacts with the E1B 19 kDa protein, which protects against virally induced cell death, as well as BCL2, another apoptotic regulator. NIP3 contains both a BH3 domain and a transmembrane domain associated with pro-apoptotic function. This dimeric mitochondrial protein can induce apoptosis even in the presence of BCL2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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