Thermo Fisher Scientific ATG5 Monoclonal Antibody (8E8G6)
ATG5 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 적합. Human 시료에 반응하며 Protein G로 정제된 액상 형태. 자가포식 및 세포사멸 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8E8G6 |
| Immunogen | Synthesized peptide of human ATG5 (aa 1–16, sequence: MTDDKDVLRDVWFGRIc) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898366 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
ATG5 (Autophagy Related 5) is a critical component in the autophagy pathway and may also play roles in apoptosis. It contributes to mitochondrial quality control, regulation of innate antiviral responses, lymphocyte development, MHC II antigen presentation, and adipocyte differentiation.
Upon conjugation with ATG12, the ATG5-ATG12 complex participates in autophagosome formation and autophagic cell death through interaction with FADD.
ATG5 expression occurs downstream of caspase activation during apoptosis and can modulate innate immune signaling by interacting with RIG-I and VISA, thereby influencing type I interferon production.
Dysfunction of ATG5 has been associated with diseases such as spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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