Thermo Fisher Scientific Phospho-PLCg1 (Tyr783) Recombinant Rabbit Monoclonal Antibody (PLCG1Y783-C4), FITC
Phospho-PLCγ1 (Tyr783) 인산화 단백질을 검출하는 FITC 결합 재조합 토끼 단일클론 항체. Flow Cytometry에 최적화되어 있으며, 높은 특이성과 일관된 로트 간 재현성을 제공합니다. 동물 유래 성분이 없는 포뮬레이션으로 연구용에 적합합니다.
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Applications
- Flow Cytometry (Flow): 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PLCG1Y783-C4 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr783 of human phospho PLCγ1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896963 |
Available Formats
- Unconjugated (MA5-37026)
- APC (MA5-37029)
- PE (MA5-37027)
- Custom conjugation available upon request
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
The mammalian Phospholipase Cγ (PLCγ) family includes two closely related proteins, PLCγ1 and PLCγ2. These isozymes share core structural domains and contain an additional PH domain, two SH2 domains, and one SH3 domain.
Upon extracellular stimulation (e.g., hormones, growth factors), receptor tyrosine kinases (RTKs) phosphorylate and activate PLCγ. Activated PLCγ catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP₂) to generate second messengers inositol 1,4,5-triphosphate (IP₃) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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