Thermo Fisher Scientific Phospho-c-Met (Tyr1234, Tyr1235) Recombinant Rabbit Monoclonal Antibody (MetY12341235-6F11), FITC
FITC 형광 표지된 recombinant rabbit monoclonal anti-phospho-c-Met (Tyr1234, Tyr1235) 항체로 인간 및 마우스 시료에 반응. Flow cytometry에 최적화되어 높은 특이성과 민감도를 제공. Protein A/G 정제 및 PBS 버퍼에 보존, 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-c-Met (Tyr1234, Tyr1235) Recombinant Rabbit Monoclonal Antibody (MetY12341235-6F11), FITC
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL / 1×10⁶ cells
Species Reactivity
- Human
- Mouse
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MetY12341235-6F11 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr1234/Tyr1235 of human phospho Met |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896971 |
Additional Formats
- Unconjugated: MA5-37034
- APC: MA5-37037
- PE: MA5-37035
- Custom conjugation available upon request
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal production candidates.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
MET (c-Met) is a receptor-like tyrosine kinase implicated in various human malignancies. Upon ligand activation, MET interacts with PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3, triggering signaling cascades such as RAS-ERK, PI3K-AKT, and PLCγ-PKC.
It plays essential roles in:
- Embryonic development (gastrulation, muscle and neuron development, angiogenesis, kidney formation)
- Adult processes (wound healing, organ regeneration, tissue remodeling)
MET dysregulation is associated with cancers including gastric, renal, and breast, making it a critical target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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