Thermo Fisher Scientific Kir2.1/KCNJ2 (extracellular) Polyclonal Antibody
Kir2.1/KCNJ2 단백질의 세포외 루프를 표적으로 하는 Thermo Fisher Scientific의 폴리클로날 항체입니다. Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 인간, 생쥐, 랫트 반응성이 있으며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Kir2.1/KCNJ2 (extracellular) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 2.5 µg |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)DLDASKESKA(S)VSE, corresponding to amino acid residues 112–125 of rat Kir2.1, extracellular loop |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: Add 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
- Ships lyophilized at room temperature; store at -20°C upon arrival.
- Reconstituted solution can be stored at 4°C for up to 1 week.
- For long-term storage, aliquot and store at -20°C.
- Avoid multiple freeze/thaw cycles.
- Centrifuge antibody preparations before use (10,000 × g, 5 min).
Target Information
The KIR2 subunit family includes 2.1, 2.2, 2.3, and 2.4. Unlike G protein-coupled KIR3 subunits, KIR2.1 requires phosphorylation by PKA and ATP hydrolysis for functional activity.
KIR2.1 is expressed in the superior and inferior colliculi and the pontine region of the brain, moderating synaptic transmission.
In the placenta, KIR2.1 is expressed throughout gestation in cytotrophoblast cells.
In the kidney, KIR2.1 colocalizes with KIR5.1 in the proximal tubule.
KIR2.1, 2.2, and 2.3 associate with the membrane-associated guanylate kinase synapse-associated protein 97 (SAP97) in the cerebellum and heart.
Phosphorylation of KIR2.2 by protein kinase A inhibits its association with SAP97.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(해당 제품 관련 이미지 파일: APC-159-200UL_Kir2.1_KCNJ2_Q64273-1_Rabbit.svg, APC-159-200UL_Kir2.1_KCNJ2_Q64273-1_Rabbit_PDP.jpeg)
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