Thermo Fisher Scientific NSE gamma (Neuron Specific Enolase, gamma) Monoclonal Antibody (ENO2, 2706)
Neuron Specific Enolase(NSE, ENO2, gamma) 단백질을 인식하는 mouse monoclonal 항체. 인간 시료에 반응하며, IHC(P)에서 1–2 µg/mL로 사용 가능. 신경세포 및 신경내분비세포 마커로 활용. Protein A/G 정제, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ENO2, 2706 |
| Immunogen | A synthetic peptide of human NSE gamma (around aa416–433) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Neuron specific enolase (NSE, also known as ENO1, ENO2, ENO3 or enolase alpha, beta, gamma) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. NSE is stable in biological fluids and diffuses into extracellular medium and cerebrospinal fluid (CSF) upon neuronal injury.
Expression:
- Alpha-subunit: most tissues
- Beta-subunit: muscle
- Gamma-subunit: neurons and neuroendocrine cells
Clinical relevance:
- Co-expressed with chromogranin A in neuroendocrine neoplasms
- Marker for neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells
- Biomarker for neuronal injury (e.g., stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, herpetic encephalitis)
- Elevated in plasma of patients with neuroblastoma and small cell lung cancer
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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