Thermo Fisher Scientific Ly-49G2 Monoclonal Antibody (eBio4D11 (4D11)), FITC, eBioscience

Thermo Fisher Scientific Ly-49G2 Monoclonal Antibody (eBio4D11 (4D11)), FITC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: View 10 publications

Product Specifications

Additional Formats

• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 4D11 monoclonal antibody reacts with Ly-49G2 (also known as LGL-1). Ly-49 family members are C-type lectin superfamily NK-cell receptors that bind MHC class I alloantigens. Ly-49G2 functions as an inhibitory receptor expressed on subsets of NK cells, NK-T cells, and memory CD8+ T cells in C57BL/6 mice.

4D11 antibody cross-reacts with Ly-49AB6, Ly-49ABALB, Ly-49T129/J inhibitory receptors, and Ly-49LCBA/J activating receptors. Expression of Ly-49A and Ly-49G2 is higher in thymic NK-T cells than in liver NK-T cells, and down-regulation of Ly-49 receptors is necessary for normal NK-T-cell development.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes
Recommended Usage: ≤ 0.25 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and help prevent NK cells from attacking normal cells while enabling attack on infected or transformed cells with down-regulated MHC class I. These inhibitory receptors are also expressed on subsets of mouse CD8+ T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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