Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), FITC, eBioscience

Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), FITC, eBioscience

Applications

• Reported Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 4 publications

Product Specifications

Additional Formats Available

• APC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family.
HLA-A3 exists as two subtypes, HLA-A3.1 and HLA-A3.2, differing at amino acid positions 152 and 156.
MHC class I molecules are expressed on all nucleated cells and are highly polymorphic cell surface glycoproteins that associate non-covalently with β2-microglobulin.
They present intracellular peptides on the cell surface, enabling recognition by cytotoxic T lymphocytes.

HLA-A3 is expressed by 15–25% of cancer patients and is known to bind the tumor-associated antigen human telomerase reverse transcriptase (hTERT).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells

• Recommended use: 5 µL (0.5 µg)/test
• Each test defined as staining a sample in 100 µL final volume
• Suitable for 10⁵–10⁸ cells/test

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues.
This molecule is a heterodimer consisting of a heavy chain and a light chain (β2-microglobulin).
The heavy chain (~45 kDa) is membrane-anchored and encoded by a gene containing 8 exons.

• Exon 1: leader peptide
• Exons 2–3: α1 and α2 domains (peptide binding)
• Exon 4: α3 domain
• Exon 5: transmembrane region
• Exons 6–7: cytoplasmic tail

Polymorphisms in exons 2 and 3 determine peptide-binding specificity.
Typing of these polymorphisms is routinely performed for bone marrow and kidney transplantation.
Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies play a key role in Immunopeptidomics, aiding identification of neoantigens via LC–MS/MS analysis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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