Thermo Fisher Scientific MEK1 Recombinant Superclonal Antibody (22HCLC)
Recombinant Superclonal™ rabbit antibody targeting human MEK1. Offers high sensitivity and monoclonal-level specificity with lot-to-lot consistency. Validated for WB, ICC/IF, and Flow Cytometry. Suitable for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 2–4 µg | - |
| Flow Cytometry (Flow) | 2–4 µg / 1×10⁶ cells | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Clone | 22HCLC |
| Immunogen | Peptide corresponding to amino acids 31–50 of human MEK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at –20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532717 |
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They combine multiple recombinant monoclonal antibodies, providing the sensitivity of polyclonal antibodies and the specificity of monoclonal antibodies, with the consistency of recombinant production.
Each lot contains a defined mixture of light and heavy chains, eliminating biological variability typical of polyclonal antibodies.
Formerly known as “Recombinant Polyclonal Antibody”, now rebranded as “Recombinant Superclonal™ Antibody”. The product and performance remain unchanged.
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in MAPK cascades, regulating cell growth and differentiation. It is activated by growth factors, cytokines, membrane depolarization, and calcium influx.
Phosphorylation of serine 218 and 222 residues by Raf1 activates MEK1. It is involved in stress response signaling, apoptosis, and cytokine-induced proliferation.
Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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