Thermo Fisher Scientific Calcineurin B Polyclonal Antibody
Calcineurin B 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합. Human, Mouse, Rat 시료 반응성. 단백질 A 정제, 1 mg/mL 농도, PBS/BSA/glycerol buffer. 연구용으로만 사용.
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- PA5144634
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:600 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within Human Calcineurin B aa 1–170/170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3094038 |
Product Specific Information
- Positive control: Rat brain tissue lysate, mouse brain tissue lysate, SiHa, rat brain tissue, HepG2
- Predicted band size: 19 kDa
- Subcellular Location: Cell membrane, sarcolemma, cytosol
Target Information
Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway.
It is composed of two subunits:
- Calcineurin A (CnA): 60 kDa catalytic subunit, homologous to protein phosphatases 1 and 2A
- Calcineurin B (CnB): 19 kDa regulatory subunit containing four EF-hand calcium binding sites
Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells.
The immunosuppressive drugs cyclosporin A and FK506, when bound to immunophilins (cyclophilin and FKBP respectively), inhibit NF-AT activation by blocking substrate access to the catalytic domain of CnA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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