Thermo Fisher Scientific PF4 Polyclonal Antibody, PeproTech
Human PF4(CXCL4)에 특이적인 Rabbit Polyclonal Antibody로, Western Blot, ELISA 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 혈소판 인자 4 연구 및 염증, 응고 관련 연구에 활용 가능.
- 카탈로그번호
- 500-P05-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific PF4 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific PF4 Polyclonal Antibody (PeproTech)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 4 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View publications |
| Immunoprecipitation (IP) | – | View 1 publication |
| Neutralization (Neu) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
| Immunostaining (IS) | – | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human PF-4 (CXCL4) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929159 |
Product Specific Information
AA Sequence of recombinant protein:
EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLESPreparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography employing an immobilized Human PF-4 matrix.Sandwich ELISA:
To detect Human PF-4 (CXCL4) by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PF-4 (CXCL4) (500-P05BT) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human PF-4 (CXCL4).Western Blot:
To detect Human PF-4 (CXCL4) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
Platelet factor-4 (PF4) is a 70-amino acid protein released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role is neutralization of heparin-like molecules on endothelial surfaces, inhibiting antithrombin III activity and promoting coagulation. PF4 also acts as a chemoattractant for neutrophils and fibroblasts, playing a role in inflammation and wound repair (Eisman et al., 1990).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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