Thermo Fisher Scientific PF4 Polyclonal Antibody, PeproTech

Thermo Fisher Scientific PF4 Polyclonal Antibody (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• AA Sequence of recombinant protein:
  EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES

• Preparation:
  Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography employing an immobilized Human PF-4 matrix.

• Sandwich ELISA:
  To detect Human PF-4 (CXCL4) by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PF-4 (CXCL4) (500-P05BT) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human PF-4 (CXCL4).

• Western Blot:
  To detect Human PF-4 (CXCL4) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Platelet factor-4 (PF4) is a 70-amino acid protein released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role is neutralization of heparin-like molecules on endothelial surfaces, inhibiting antithrombin III activity and promoting coagulation. PF4 also acts as a chemoattractant for neutrophils and fibroblasts, playing a role in inflammation and wound repair (Eisman et al., 1990).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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