Thermo Fisher Scientific PF4 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 4 publications 4 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Immunostaining (IS)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human PF-4 (CXCL4) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PF4,uniProtId:P02776-1,ncbiNodeId:9606,antigenRange:32-101,antigenLength:101,antigenImageFileName:500-P05-100UG_PF4_P02776-1_Rabbit.svg,antigenImageFileNamePDP:500-P05-100UG_PF4_P02776-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929159

Product Specific Information

AA Sequence of recombinant protein: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography employing an immobilized Human PF-4 matrix.

Sandwich ELISA: To detect Human PF-4 (CXCL4) by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PF-4 (CXCL4) (500-P05BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human PF-4 (CXCL4).

Western Blot: To detect Human PF-4 (CXCL4) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.