Thermo Fisher Scientific PF4 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human PF-4 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PF-4 (500-P05) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human PF-4.

Western Blot:
To detect hPF-4 by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hPF-4 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P05BT-1MG will be provided as 2 × 500 µg.

Target Information

Platelet factor-4 (PF4) is a 70-amino acid protein released from the alpha-granules of activated platelets. It binds with high affinity to heparin and neutralizes heparin-like molecules on the endothelial surface, inhibiting local antithrombin III activity and promoting coagulation. PF4 also acts as a chemoattractant for neutrophils and fibroblasts, suggesting roles in inflammation and wound repair (Eisman et al., 1990).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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