Thermo Fisher Scientific SPAG5 Monoclonal Antibody (OTI5B2), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST, or Astrin, is a 1,193 amino acid protein highly expressed in testis and at lower levels in liver, placenta, pancreas, colon, and thymus.
It localizes to spindle poles during prophase and throughout the spindle during metaphase and anaphase, playing a crucial role in spindle formation and mitotic regulation.
SPAG5 also contributes to sister chromatid cohesion and separation, maintaining chromosome integrity through its association with chromatids.
It forms a homodimer with a long stalk and globular head domain, exhibiting aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.