Thermo Fisher Scientific SPAG5 Monoclonal Antibody (OTI5H5), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform additional desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST, or Astrin, is a 1,193 amino acid protein highly expressed in testis and at lower levels in liver, placenta, pancreas, colon, and thymus.
It localizes to spindle poles during prophase and throughout the spindle during metaphase and anaphase, playing an essential role in spindle formation and mitotic regulation.
SPAG5 contributes to sister chromatid cohesion and separation, maintaining chromosome integrity. It forms homodimers with a long stalk and globular head, producing aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.