Thermo Fisher Scientific MARK3 Monoclonal Antibody (OTI2D9), TrueMAB
인간 MARK3 단백질을 인식하는 마우스 단클론 항체. Western blot 및 IHC(Paraffin) 실험에 적합. 동결건조 형태로 제공되며, PBS 버퍼와 트레할로오스 포함. 연구용으로 세포 극성 및 미세소관 관련 단백질 인산화 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 473–729 of human MARK3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7 K MWCO, 0.5 mL, Product #89882).
Target Information
MARK3 is a kinase involved in regulating cell polarity through phosphorylation of microtubule-associated proteins (MAPs). It is activated by phosphorylation and participates in the phosphorylation of tau proteins MAP2 and MAP4. Multiple transcript variants encoding different isoforms have been identified for MARK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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