Thermo Fisher Scientific Mouse IgG3 Isotype Control (B10), eBioscience

Applications

Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgG3, lambda

Class

Monoclonal

Type

Isotype Control

Clone

B10

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865073

Product Specific Information

Description: The mouse IgG3 lambda is useful as an isotype control immunoglobulin.

Applications Reported: This mouse IgG3 isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.

Applications Tested: This mouse IgG3 isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells. Use the isotype control at the same concentration as the experimental antibody.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgG3 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the least abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG3 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG3 is less commonly used in research and diagnostic applications compared to other IgG subtypes, but it is still used in some applications such as ELISA and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.