Thermo Fisher Scientific beta-3 Tubulin Monoclonal Antibody (2G10-TB3), eBioscience
신경세포 특이적 마커인 beta-3 Tubulin을 인식하는 단클론 항체. Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능. 사람, 생쥐, 쥐, 소에 반응. 고순도(>90%) 항체로 0.5 mg/mL 농도의 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2.5–5 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 4 publications |
| Flow Cytometry (Flow) | - | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2G10-TB3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865070 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats Available
Product Specific Information
The 2G10 monoclonal antibody recognizes beta tubulin class III, a component of microtubules in the cytoskeletal network. Beta tubulin class III expression is found exclusively in neuronal cells of adult mammals. During fetal development, transient expression occurs in both glial and neuronal precursors in the subventricular zone.
Identification of beta tubulin class III is useful as a structural marker for developing and mature neural cells. Due to the high conservation of this protein, clone 2G10 is expected to recognize human, mouse, rat, and bovine beta tubulin class III.
Applications Reported: Immunoprecipitation, Western blotting, immunohistochemical staining (frozen and paraffin-embedded tissues), and immunocytochemistry.
Applications Tested: Suitable for immunocytochemistry at ≤5 µg/mL. Optimize concentration for best results.
Target Information
BetaIII-tubulin is a neuronal marker and one of the earliest indicators of neuronal differentiation. It serves as a specific probe for neuronal cells and tumors derived from them. Although primarily found in neuronal cells, it is also detected in Sertoli cells of the testis and transiently in some non-neuronal embryonic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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