Thermo Fisher Scientific AHR Monoclonal Antibody (4MEJJ), PE, eBioscience

Applications

Immunohistochemistry (PFA fixed) (IHC (PFA))

• Tested Dilution: Not specified
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 1 µg/test
• Publications: View 5 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• eFluor 660
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

This 4MEJJ monoclonal antibody recognizes mouse aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor mediating responses to environmental contaminants such as PCBs and TCDD. AHR also binds naturally occurring compounds found in fruits, vegetables, and metabolic byproducts. It is localized in the cytoplasm in complex with HSP90, p23, and XAP2/AIP/ARA9. Upon ligand binding, AHR translocates to the nucleus and forms a complex with ARNT to regulate gene expression, including CYP1A1.

The antibody recognizes b-1, b-2, and d alleles of mouse AHR but not human AHR.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on 3-day Th17-polarized mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00). Recommended ≤1 µg/test (100 µL final volume). Optimal titration is advised.

Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

AHR (Ah Receptor) is a ligand-activated transcription factor belonging to the PAS domain family, including ARNT (HIF-1 Beta). It binds specific DNA enhancer sequences to regulate cytochrome P450 (CYP1A1) gene expression. AHR mediates responses to xenobiotic compounds such as polycyclic and halogenated aromatic hydrocarbons (e.g., TCDD). In non-ligand-activated cells, AHR is complexed with HSP90 in the cytoplasm. Upon activation, it translocates to the nucleus, forms a heterodimer with ARNT, and binds DNA to regulate gene transcription. Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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