Thermo Fisher Scientific Ly-6K Monoclonal Antibody (B33fc8k), PE, eBioscience
마우스 Ly-6K 단백질을 인식하는 B33fc8k 단클론 항체로, PE 형광체가 결합되어 흐름세포분석(Flow Cytometry)에 최적화됨. 488–561 nm 파장에서 여기되며, 578 nm에서 방출. 0.2 mg/mL 농도의 액상 제품으로 4°C 암소 보관. 연구용 전용 시약.
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Thermo Fisher Scientific Ly-6K Monoclonal Antibody (B33fc8k), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1 µg/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B33fc8k |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11149356 |
Product Specific Information
This B33fc8k monoclonal antibody reacts with mouse Ly-6k, a member of the Ly6/uPAR superfamily. Ly-6k is a GPI-linked protein predominantly found on the cell surface, with possible non-membranous expression. It is expressed on B cells, plasma cells, and testis cells.
Using the B33fc8k antibody, Ly-6k expression is observed on a B lymphocyte subset in the spleen corresponding to transitional cells (CD23-IgM-).
In humans, Ly-6k is associated with carcinomas of the head and testis.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes; use ≤1 µg per test (100 µL final volume). Cell number can range from 10⁵–10⁸ cells/test. Antibody should be titrated for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ly-6K is required for sperm migration into the oviduct and male fertility by controlling sperm binding to the zona pellucida. It may also play a role in cell growth (PubMed:18089789). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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