Thermo Fisher Scientific E4BP4 (NFIL3) Monoclonal Antibody (S2M-E19), PE, eBioscience
마우스 E4BP4(NFIL3)을 인식하는 단클론항체로, PE 형광표지되어 유세포분석(Flow Cytometry)에 적합합니다. 세포 내 염색용으로 최적화되었으며, NK/NKT 세포 활성화 후 발현 연구에 활용 가능합니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Intracellular Staining)
- Tested Dilution: ≤ 0.25 µg/test
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S2M-E19 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11150965 |
Additional Formats
Product Specific Information
The S2M-E19 monoclonal antibody recognizes mouse E4BP4 (NFIL3), a basic leucine zipper transcription factor first identified for its binding to the promoters of the adenovirus E4 protein and human IL-3.
E4BP4 is involved in:
- Transcriptional control of the mammalian circadian clock
- Motor neuron growth and survival
- Regulation of von Willebrand factor expression
- Osteoblast function
It is also required for the development of NK and NKT cells as well as CD8α+ dendritic cells.
Although relatively high levels of mRNA are reported in resting NK and NKT cells, eBioscience studies observed E4BP4 protein expression in lymphocytes only after activation.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
Applications Tested:
- Tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00)
- Recommended use: ≤ 0.25 µg per test (100 µL final volume; 10⁵–10⁸ cells/test)
- Careful titration recommended for optimal performance
Excitation / Emission / Laser:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Expression of interleukin-3 (IL3) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines.
Transcription initiation depends on activating protein factors such as NFIL3, which bind to regulatory regions upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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