Thermo Fisher Scientific LSD1 Monoclonal Antibody (1E5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E5

Immunogen

Purified recombinant human KDM1A protein fragments expressed in E.coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.2% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2717313

Product Specific Information

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

LSD1 is a histone demethylase that specifically demethylates Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys-4 of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys-4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate Lys-9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.