Thermo Fisher Scientific Caspase 1 p20 (Cleaved Asp296) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CASP1(Accession P29466), corresponding to amino acid residues I280-V299. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Caspase 1 p20,uniProtId:P29466-1,ncbiNodeId:9606,antigenRange:280-299,antigenLength:404,antigenImageFileName:PA5-99390_Caspase_1_p20_P29466-1_Rabbit.svg,antigenImageFileNamePDP:PA5-99390_Caspase_1_p20_P29466-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2818323

Product Specific Information

Antibody detects endogenous levels of fragment of activated Caspase-1 resulting from cleavage adjacent to Asp296.

Target Information

Caspase-1 (CASP1) is a thiol protease that cleaves IL-1 beta between Asp and Ala. This cleavage causes the release of the mature cytokine which is involved in a variety of inflammatory processes. CASP1 is important for defense against pathogen. It cleaves and activates sterol regulatory element binding proteins (SREBPs). CASP1 also promotes apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, CASP1 controls antiviral immunity through the cleavage of CGAS and renders it inactive.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.