Thermo Fisher Scientific Phospho-ASK1 (Ser83) Polyclonal Antibody
인간 ASK1 단백질의 Ser83 인산화 형태를 특이적으로 검출하는 폴리클로나 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며 고순도 정제된 액상형 제품입니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAP3K5 (Accession Q99683), corresponding to amino acid residues around phosphorylated Ser83 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2818322 |
Product Specific Information
This antibody detects endogenous levels of ASK1 only when phosphorylated at Serine 83.
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas. It is activated by extracellular stimuli such as cytokines, growth factors, and environmental stresses. ASK1 (also known as MAPKKK5) activates MKK4 and MKK6, which subsequently activate JNK and p38 MAP kinases. Activation occurs through TNFR and Fas via TRAF family members and Daxx. Overexpression of ASK1 induces apoptotic cell death, while inactive ASK1 inhibits TNF-α-induced apoptosis. ASK1 is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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