Thermo Fisher Scientific Phospho-IkappaB beta (Ser19) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of IKB beta only when phosphorylated at Ser19.

Target Information

The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB.
The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.
IKK alpha and IKK beta serve as the catalytic subunits of the kinase, and IKK gamma serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKK beta (Ser176 and Ser180 in IKK alpha), which causes conformational changes resulting in kinase activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지: PA5-99388_IkB_beta_Q15653-1_Rabbit_PDP.jpeg)