Thermo Fisher Scientific RPS6KA5/MSK1 Monoclonal Antibody (PCRP-RPS6KA5-1A8)
인간 RPS6KA5/MSK1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체입니다. Western blot, ICC/IF, Flow cytometry 등 다양한 응용에 적합하며, 단백질 A/G로 정제된 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific RPS6KA5/MSK1 Monoclonal Antibody (PCRP-RPS6KA5-1A8)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-RPS6KA5-1A8 |
| Immunogen | Recombinant full-length human RPS6KA5 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
This antibody is predicted to react with Mouse and Rat.
Target Information
RPS6KA5 (MSK1) is a serine/threonine protein kinase essential for regulating various cellular processes. It mediates mitogen- or stress-induced phosphorylation of transcription factors such as CREB1 and ATF1 and regulates RELA, STAT3, and ETV1/ER81. It contributes to gene activation via histone phosphorylation and inflammatory gene regulation.
MSK1 phosphorylates CREB1 and ATF1 in response to stimuli such as UV-C irradiation, EGF, and anisomycin. It also controls RELA transcriptional activity in response to TNF and glucocorticoids through cytoplasmic association with NR3C1 (glucocorticoid receptor), repressing inflammatory gene expression.
In skeletal myoblasts, MSK1 is required for RELA phosphorylation at Ser-276 during oxidative stress, and in erythropoietin-stimulated cells, it mediates STAT3 Ser-727 phosphorylation and transcriptional regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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