Thermo Fisher Scientific ATP1A3 Monoclonal Antibody (XVIF9-G10)
ATP1A3 단백질의 alpha-3 서브유닛을 특이적으로 인식하는 Thermo Fisher Scientific의 단일클론 항체. Western blot, IHC 등 다양한 응용에 적합하며, Protein A로 정제된 액상 형태로 제공. 여러 종에서 반응성이 확인된 연구용 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 65 publications |
| Immunohistochemistry (IHC) | - | 19 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 3 µg/mL | 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 12 publications |
| Flow Cytometry (Flow) | 1 µg / 10^6 cells | - |
| Immunoprecipitation (IP) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Guinea pig, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat |
| Published Species | Amphibian, Bovine, Gerbil, Human, Mouse, Non-human primate, Pig, Rat, Shark |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | XVIF9-G10 |
| Immunogen | Canine cardiac microsomes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2274447 |
Product Specific Information
MA3-915 detects sodium/potassium ATPase from human, monkey, bovine, sheep, canine, rabbit, guinea pig, mouse, and rat tissue. This antibody is specific for the alpha-3 subunit.
MA3-915 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~110 kDa protein representing the alpha-3 subunit of the sodium/potassium ATPase from canine skeletal muscle extract. Immunohistochemical staining of sodium/potassium ATPase in rat retina with MA3-915 yields a pattern consistent with plasma membrane localization.
The MA3-915 antigen is canine cardiac microsomes.
Target Information
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This enzyme is related to other ATPases, including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA).
The sodium/potassium ATPase consists of:
- A large, multipass, transmembrane catalytic subunit (alpha subunit)
- An associated smaller glycoprotein (beta subunit)
Studies indicate:
- Three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3)
- Two isoforms of the beta subunit (beta 1, beta 2)
- Isoforms exhibit tissue-specific and developmental expression patterns
The alpha-3 subunit is found in neuronal, skeletal, and cardiac muscle, as well as lung and stomach tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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