Thermo Fisher Scientific ATP1A3 Monoclonal Antibody (XVIF9-G10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 65 publications 65 publications

Immunohistochemistry (IHC)

-

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

3 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 12 publications 12 publications

Flow Cytometry (Flow)

1 µg / 10^6 cells

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Guinea pig, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat

Published species

Amphibian, Bovine, Gerbil, Human, Mouse, Non-human primate, Pig, Rat, Shark

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

XVIF9-G10

Immunogen

Canine cardiac microsomes.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2274447

Product Specific Information

MA3-915 detects sodium/potassium ATPase from human, monkey, bovine, sheep, canine, rabbit, guinea pig, mouse and rat tissue. This antibody is specific for the alpha-3 subunit.

MA3-915 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~110 kDa protein representing the alpha-3 subunit of the sodium/potassium ATPase from canine skeletal muscle extract. Immunohistochemical staining of sodium/potassium ATPase in rat retina with MA3-915 yields a pattern consistent with plasma membrane localization.

The MA3-915 antigen is canine cardiac microsomes.

Target Information

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.