Thermo Fisher Scientific Phospho-TRIM28 (Ser824) Recombinant Rabbit Monoclonal Antibody (JE50-99)
Phospho-TRIM28 (Ser824) 인산화 단백질을 특이적으로 인식하는 재조합 토끼 단일클론 항체. Western blot, IHC(P), Dot blot 등에 사용 가능. 단백질 A로 정제된 액상 항체로, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Dot Blot (DB) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE50-99 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser824 of human KAP1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2898927 |
Product Specific Information
This product is predicted to work in Immunoprecipitation at a dilution of 1:10–1:50.
Target Information
The KRAB (Kruppel-associated box) domain is approximately 45 amino acids in length and functions as a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription through specific interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency and interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 targets KRAZ1 to centromeric heterochromatin foci containing HP1alpha, regulating transcriptional repression. Mutations in KAP-1 that bind KRAB but not HP1 lead to random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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