Thermo Fisher Scientific TGF beta-1,2,3 Monoclonal Antibody (1D11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Neutralization (Neu)

0.25-1.25 µg/mL

View 3 publications 3 publications

Inhibition Assays (IA)

Assay-Dependent

View 3 publications 3 publications

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D11

Immunogen

Bovine bone-derived TGF-beta 1 and TGF-beta 2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TGF beta-1,2,3,uniProtId:P18341-1,ncbiNodeId:9913,antigenRange:1-390,antigenLength:390,antigenImageFileName:MA5-23795_TGF_beta-1,2,3_P18341-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23795_TGF_beta-1,2,3_P18341-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609812

Product Specific Information

This antibody recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Neutralization: measured by its ability to neutralize TGF beta-1 inhibition of IL-4-dependent proliferation in the HT-2 mouse T cell line. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Recombinant Human TGF beta-1 and 7.5 ng/mL Recombinant Mouse IL-4.

Target Information

TGF-beta1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta1, -2, and -3 are disulfide-linked homodimers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.