Thermo Fisher Scientific Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody (VM452), CF 488
중간엽 세포 마커인 Vimentin을 인식하는 Thermo Fisher Scientific의 단클론 항체. CF® 488 형광 표지로 ICC/IF 및 유세포분석에 적합. 다종 반응성(인간, 개, 돼지 등) 보유. 단백질 A/G 정제, 액상 형태, 4°C 보관.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50–1:100
Flow Cytometry (Flow)
- Tested Dilution: 5 µL/10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Cat, Goat, Human, Pig |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VM452 |
| Immunogen | Recombinant human vimentin protein |
| Conjugate | CF® 488 |
| Excitation / Emission Max | 488 / 515 nm |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
- Does not react with Mouse or Rat
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular protein transport between the nucleus and plasma membrane. Unlike other IFPs, vimentin is expressed along with desmin during early cellular development and later replaced by tissue-specific IFPs.
Vimentin plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to intermediate filament breakdown and activation of ATP- and myosin light chain-dependent contractile events, facilitating lipid droplet interaction with mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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