Thermo Fisher Scientific Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody (rVIM/6914)
인간 Vimentin 단백질을 인식하는 마우스 단클론 항체로, 중간엽 세포 마커 연구에 적합합니다. Western blot, IHC(P), IHC(PFA) 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 형태이며, 보존제 없이 PBS(pH 7.4)에 저장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | rVIM/6914 |
| Immunogen | Recombinant fragment (around aa366–466) of human Vimentin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFPs, vimentin is expressed with desmin during early cellular development and later replaced by tissue-specific IFPs.
Vimentin plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to intermediate filament breakdown and activation of contractile events that facilitate lipid droplet interaction with mitochondria, enhancing steroid synthesis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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