Thermo Fisher Scientific Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody (VM452)
Mesenchymal cell marker로 사용되는 Vimentin 단일클론 항체로, 다양한 종에 반응하며 WB, IHC, Flow Cytometry 등 다중 응용이 가능. Recombinant full-length human vimentin을 면역원으로 사용. 단백질 A/G 정제, 액상 형태, PBS buffer에 보존.
- 카탈로그번호
- 7431-MSM1-P1ABX
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Cat, Goat, Human, Pig |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VM452 |
| Immunogen | Recombinant full-length human vimentin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- CF 488 conjugate available
- Custom conjugation upon request
Product Specific Information
- Does not react with Mouse or Rat
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular protein transport between the nucleus and plasma membrane. Vimentin is co-expressed with desmin during early cellular development and later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin induces cytoskeletal changes that facilitate cholesterol transport to mitochondria, enhancing steroidogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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