Thermo Fisher Scientific CD21 Monoclonal Antibody (HB5), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

The HB5 monoclonal antibody reacts with human CD21, a ~145 kDa type I transmembrane protein expressed by mature B cells, follicular dendritic cells, and a subset of epithelial cells. CD21 acts as a receptor for complement component C3d and Epstein-Barr virus (EBV), and participates in B cell activation through association with CD19 and CD81.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Use at 5 µL (0.02 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability with NovaFluor dyes.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample (regardless of number of NovaFluors).
  • For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for minimal spillover and optimal spectral separation.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments and plays a role in immune complex uptake, memory B cell survival, and humoral immune response development. It also serves as a receptor for Epstein-Barr virus and is involved in prion targeting to follicular dendritic cells. Soluble CD21 (sCD21) retains ligand-binding ability. CD21 associates with CD19, CD81, and Leu13 to form a signal transduction complex important for B cell activation. Genetic variations are linked to susceptibility to systemic lupus erythematosus type 9 (SLEB9).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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