Thermo Fisher Scientific CD21 Monoclonal Antibody (HB5), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.2 µg)/test

Product Specifications

Additional Available Formats

• APC
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

The HB5 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein.
CD21 is expressed by mature B cells, follicular dendritic cells, and a subset of epithelial cells, serving as a receptor for complement component C3d and Epstein-Barr virus (EBV).
In association with CD19 and CD81, CD21 participates in a multimolecular complex with BCR and is involved in B cell activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (0.02 µg) per test in a final volume of 100 µL. Cell number: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample, regardless of the number of NovaFluors in your panel.
  • For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, utilizing novel nucleic acid dye structures engineered for optimized fluorescent signatures with reduced spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD21 (Complement Receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, especially breakdown fragments covalently attached to complement-activating surfaces or antigens.
It plays critical roles in:

• Uptake and retention of immune complexes
• Survival of memory B cells
• Maintenance of humoral response to T-dependent antigens
• Acting as a receptor for Epstein-Barr virus and prions

A soluble form (sCD21) is shed from lymphocytes and retains ligand-binding ability.
CD21 associates with CD19, CD81, and Leu13 in a signal-transduction complex involved in B cell activation.
Genetic variations in CD21 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).
Alternative splicing generates different isoforms of CD21.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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