Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Alexa Fluor 488 (53-7321-82)
• Alexa Fluor 561 (505-7321-82)
• Alexa Fluor 660 (606-7321-82)
• APC (17-7321-82)
• Brilliant Violet 421 (404-7321-82)
• Brilliant Violet 480 (414-7321-82)
• Brilliant Violet 605 (406-7321-82)
• Brilliant Violet 650 (416-7321-82)
• Brilliant Violet 786 (417-7321-82)
• eFluor 450 (48-7321-82)
• FITC (11-7321-82)
• Functional Grade (16-7321-81)
• PE (12-7321-82)
• PE-Cyanine7 (25-7321-82)
• PE-eFluor 610 (61-7321-82)
• PerCP-eFluor 710 (46-7321-82)
• Request custom conjugation

Product Specific Information

The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4(+) T cells. TNF alpha has cytolytic activity against various tumor cells and plays an important role in immune regulation. It exists in soluble (17 kDa) and membrane-bound (26 kDa) forms, forming dimers and trimers.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis
• Immunohistology staining of frozen tissue sections
  (Fluorochrome-conjugated MP6-XT22 is recommended for intracellular flow cytometry.)

Applications Tested:

• Tested to block staining with fluorochrome-conjugated MP6-XT22
• Recommended ≤ 2 µg/test (in 100 µL final volume)
• Optimal titration required for specific assays
• Suitable for 10⁵–10⁸ cells/test

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cell function, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It forms multimers (dimers, trimers, pentamers) and shows a single 17 kDa band under non-reducing SDS-PAGE conditions.

Knockout mouse studies indicate TNF alpha’s neuroprotective role and tumor necrosis induction in tumor-bearing mice. It also contributes to immune responses against bacterial, viral, parasitic, and fungal infections and participates in tumor cytotoxicity, synergistic with IFN-gamma. TNF alpha is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.