Thermo Fisher Scientific DNMT3B Polyclonal Antibody
DNMT3B 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, ICC/IF, ELISA에 적합하며, 핵 내 DNMT3B 발현 연구에 유용합니다. -20°C 보관, glycerol 포함 PBS buffer 포맷입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 345–475 of human DNMT3B (NP_008823.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.88 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806309 |
Product Specific Information
- Positive Samples: U-251MG, HCT116, mouse testis, C6
- Cellular Location: Nucleus
- Immunogen sequence:
IEGLKPNNTQ PVVNKSKVRR AGSRKLESRK YENKTRRRTA DDSATSDYCP APKRLKTNCY NNGKDRGDED QSREQMASDV ANNKSSLEDG CLSCGRKNPV SFHPLFEGGL CQTCRDRFLE LFYMYDDDGY Q
Target Information
CpG methylation is an epigenetic modification critical for embryonic development, imprinting, and X-chromosome inactivation. DNA methylation is essential for mammalian development.
This gene encodes a DNA methyltransferase involved in de novo methylation, primarily localized in the nucleus, with developmentally regulated expression.
Mutations in this gene are associated with ICF syndrome (Immunodeficiency-Centromeric instability-Facial anomalies).
Six alternatively spliced transcript variants have been described, though the full-length sequences of variants 4 and 5 have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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