Thermo Fisher Scientific DNMT3B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 345-475 of human DNMT3B (NP_0088231) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DNMT3B,uniProtId:Q9UBC3-1,ncbiNodeId:9606,antigenRange:345-475,antigenLength:853,antigenImageFileName:PA5-91864_DNMT3B_Q9UBC3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-91864_DNMT3B_Q9UBC3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.88 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806309

Product Specific Information

Positive Samples: U-251MG, HCT116, mouse testis, C6; Cellular Location: Nucleus

Immunogen sequence: IEGLKPNNTQ PVVNKSKVRR AGSRKLESRK YENKTRRRTA DDSATSDYCP APKRLKTNCY NNGKDRGDED QSREQMASDV ANNKSSLEDG CLSCGRKNPV SFHPLFEGGL CQTCRDRFLE LFYMYDDDGY Q

Target Information

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.