Thermo Fisher Scientific EGR-1 Polyclonal Antibody
EGR-1 단백질을 인식하는 Thermo Fisher의 폴리클로날 항체로, WB, IHC, ELISA, IP에 사용 가능. 인간, 침팬지, 마우스에 반응하며, 친화 크로마토그래피로 정제된 액상 항체. 연구용으로 안정적이며 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–20 µg/mL |
| ELISA | 1:4,000–1:16,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Chimpanzee, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity-purified antibody prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 85–115 of Human EGR-1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.93 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human EGR-1 protein. A BLAST analysis suggests reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross-react with EGR-1.
Target Information
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Studies suggest this is a cancer suppressor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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