Thermo Fisher Scientific GM-CSF Monoclonal Antibody (31G6), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

31G6

Immunogen

Recombinant mouse GM-CSF

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223605

Product Specific Information

MM500DB targets Mouse GM-CSF in WB applications and shows reactivity with mouse samples.

The MM500DB immunogen is recombinant mouse GM-CSF.

MM500DB detects Mouse GM-CSF which has a predicted molecular weight of approximately 14 kDa.

The MM500DB mouse GMCSF antibody (biotinylated conjugate of clone 31G6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM500C (clone 22E9) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody MM500DB (clone 31G6) and antibody MM500C (clone 22E9) have successfully been used in combination with recombinant mouse GMCSF protein SMGMCSF in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.