Thermo Fisher Scientific GPX1 Polyclonal Antibody
Thermo Fisher Scientific의 GPX1 Polyclonal Antibody는 인간 및 랫트 시료에서 글루타티온 퍼옥시다제 1(GPx1)을 검출하기 위한 항체입니다. Western blot 및 IHC(P/F)에 적합하며, 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:100,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:2,000–1:20,000 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:2,000–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues R(175)–L(194) of human glutathione peroxidase 1 (GPx-1) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | -20 °C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2112241 |
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution, keep aliquots at -20 °C for higher stability, and at 4 °C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for additional stability.
Target Information
Glutathione peroxidases (Gpxs) are ubiquitously expressed enzymes that catalyze the reduction of hydrogen peroxide and organic hydroperoxides by glutathione. Several isoforms exist, differing in primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme and was the first GPx family member discovered. GPx2 (GI-GPx) is expressed in the gastrointestinal epithelium, while GPx3 (pGPx) is secreted mainly by the kidney into the bloodstream. GPx4 (PH-GPx) acts on a wide range of hydroperoxides, including those in membranes and lipids. All mammalian GPx family members, except Cys-containing GPx3 and epididymis-specific GPx5, have selenocysteine at the active site.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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