Thermo Fisher Scientific Baculovirus Envelope gp64 Monoclonal Antibody (AcV1), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats:

• APC
• PE
• Request custom conjugation

Product Specific Information

Description:
The AcV1 monoclonal antibody reacts with the gp64 envelope protein of the baculovirus Autographa californica (AcMNPV). The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection. The AcV1 antibody can be used in flow cytometry-based viral titration experiments.

Applications Reported:
The AcV1 antibody has been reported for use in flow cytometric analysis and in identifying virally infected insect cells.

Applications Tested:
The AcV1 antibody has been tested by flow cytometry on baculovirus-infected insect cells. It can be used at ≤1 µg per test (defined as the amount needed to stain a cell sample in a final volume of 100 µL). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Baculoviruses infect invertebrates, with over 600 species described. Immature (larval) forms of moth species are the most common hosts, but infections have also been observed in sawflies, mosquitoes, and shrimp. Although baculoviruses can enter mammalian cells in culture, they are not known to replicate in mammalian or other vertebrate cells. The gp64 envelope protein is essential for viral infectivity and is expressed on the surface of infected cells within six hours post-infection.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.