Thermo Fisher Scientific Platelet GMP-140 (P-selectin/CD62) Monoclonal Antibody (WGA-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

5-10 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

5-10 µg/mL

-

Flow Cytometry (Flow)

1 µg/mL

-

ELISA (ELISA)

1-10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

WGA-1

Immunogen

Human platelets if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:P-Selectin (CD62P),uniProtId:P16109-1,ncbiNodeId:9606,antigenRange:1-830,antigenLength:830,antigenImageFileName:50122-100UG_P-Selectin_CD62P_P16109-1_House_mouse.svg,antigenImageFileNamePDP:50122-100UG_P-Selectin_CD62P_P16109-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5-1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 50 µL of distilled water (final concentration 2 mg/mL).

Target Information

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.