Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eFluor 660, eBioscience

Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eFluor 660, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBioH4A3 monoclonal antibody reacts with human CD107a (LAMP-1), a highly glycosylated lysosomal membrane protein (~110 kDa). It is mainly expressed intracellularly but transiently appears on the surface of degranulating cytolytic T cells, activated platelets, and certain cancer cells.

Applications Reported: Suitable for intracellular staining and flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining and flow cytometry using Jurkat cells at 5 µL (0.125 µg) per test.
A test corresponds to staining a sample in a final volume of 100 µL, with cell numbers ranging from 10⁵ to 10⁸ per test.

Fluorochrome Information:
eFluor™ 660 replaces Alexa Fluor™ 647.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

LAMP1 (CD107a) is a major lysosomal membrane component, with 1–2% found on the plasma membrane. It is a heavily glycosylated type I integral membrane protein involved in lysosome biogenesis and fusion with phagosomes. The protein’s trafficking depends on a C-terminal tyrosine motif (YXXI). Mutations affecting this motif alter lysosomal localization.

Cell surface LAMP1 promotes adhesion of PBMCs to vascular endothelium, suggesting a role in inflammation. Increased LAMP1 expression is observed around senile plaques in Alzheimer’s disease and in activated immune cells. LAMP1 is a useful marker for mast cell activation.

For Research Use Only. Not for use in diagnostic procedures.

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