Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eFluor 660, eBioscience
인간 CD107a(LAMP-1)를 인식하는 eBioH4A3 단클론 항체로, eFluor 660 형광체가 결합된 제품입니다. 세포 내 염색 및 유세포분석에 최적화되어 있으며, 높은 특이성과 안정성을 제공합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eFluor 660, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | - |
| Immunocytochemistry (ICC/IF) | - | View 7 publications |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 13 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH4A3 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11220283 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBioH4A3 monoclonal antibody reacts with human CD107a (LAMP-1), a highly glycosylated lysosomal membrane protein (~110 kDa). It is mainly expressed intracellularly but transiently appears on the surface of degranulating cytolytic T cells, activated platelets, and certain cancer cells.
Applications Reported: Suitable for intracellular staining and flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining and flow cytometry using Jurkat cells at 5 µL (0.125 µg) per test.
A test corresponds to staining a sample in a final volume of 100 µL, with cell numbers ranging from 10⁵ to 10⁸ per test.
Fluorochrome Information:
eFluor™ 660 replaces Alexa Fluor™ 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
LAMP1 (CD107a) is a major lysosomal membrane component, with 1–2% found on the plasma membrane. It is a heavily glycosylated type I integral membrane protein involved in lysosome biogenesis and fusion with phagosomes. The protein’s trafficking depends on a C-terminal tyrosine motif (YXXI). Mutations affecting this motif alter lysosomal localization.
Cell surface LAMP1 promotes adhesion of PBMCs to vascular endothelium, suggesting a role in inflammation. Increased LAMP1 expression is observed around senile plaques in Alzheimer’s disease and in activated immune cells. LAMP1 is a useful marker for mast cell activation.
For Research Use Only. Not for use in diagnostic procedures.
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