Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 737, 805
• Brilliant Violet 421, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132 (common gamma chain, γc) to form the IL-7 receptor (IL-7R).
CD127 is a type I glycoprotein (75–80 kDa) expressed by immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation.

IL-7 binding triggers signal transduction via tyrosine kinase pathways, including Jak/STAT. IL-7 is essential for lymphocyte development and homeostatic proliferation of T cells.
IL-7R signaling also regulates TCR locus rearrangement in γδ T cells.

CD127 expression is down-regulated on CD4⁺CD25⁺ regulatory T cells (Tregs). Cells with low CD127 and high CD25 are enriched for regulatory T cells expressing Foxp3 and suppressive activity.
Binding of eBioRDR5 to PBMCs is blocked by pre-incubation with recombinant human IL-7 (Product #14-1079-80).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells at 5 µL (0.06 µg)/test (100 µL final volume).
Laser/Emission: Excitation 633–647 nm; Emission 668 nm; Red laser
Filtration: 0.2 µm post-manufacturing filtered

eFluor® 660 is a replacement for Alexa Fluor® 647, excited by red laser (633 nm) and emitting at 659 nm.

Target Information

CD127 (Interleukin-7 Receptor alpha, IL7Rα) is a glycoprotein regulating lymphopoiesis. The functional receptor is an α/γ heterodimer (IL7Rα and γc).
The γc chain activates signal transduction, while the α chain mediates specific signaling events.
CD127 promotes proliferation of precursor B cells, thymocytes, and T cell subsets.
Dysfunction is associated with severe combined immunodeficiency (SCID) and related disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지