Thermo Fisher Scientific POT1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to an internal portion of the human POT1 protein sequence (between residues 200-300). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:POT1,uniProtId:Q9NUX5-1,ncbiNodeId:9606,antigenRange:200-300,antigenLength:634,antigenImageFileName:PA1-16830_POT1_Q9NUX5-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16830_POT1_Q9NUX5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2166765

Product Specific Information

The target sequence has 95% sequence homology with monkey, 82% sequence homology with rat, and 72% sequence homology with mouse.

Suggested positive control: HeLa nuclear extract, antigen standard for POT1 (transient overexpression lysate).

Target Information

POT1 is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Pot1 also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. It binds to two or more telomeric single-stranded 5-TTAGGG-3 repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5-TAGGGTTAG-3 sequence. Its activity is TERT dependent but it does not increase TERT activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.