Thermo Fisher Scientific Human IL-10 Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA (ELISA): Assay-dependent
• Immunoprecipitation (IP): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

• Use: IL-10 exerts activity in the range of 0.2 to 20 ng/mL for most in vitro applications.
• Reconstitution and Dilution:
  • Reconstitute in distilled water to a concentration of 100 µg/mL.
  • After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5–10% FBS or 1–4% high purity BSA (fatty acid free fraction V).
• Storage Stability:
  • Lyophilized product is stable until expiration if stored desiccated below 0ºC.
  • Reconstituted undiluted cytokine should be stored in working aliquots at -70ºC.
  • Cytokines diluted as indicated above should be divided into aliquots and stored at -70ºC.
  • Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes.
This cytokine regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages.
It enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway.
Knockout studies in mice have shown IL-10 is an essential immunoregulator in the intestinal tract.
Mutations in this gene are associated with increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.